|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL7 ORF mammalian expression plasmid, C-GFPSpark tag||RG80329-ACG|
|Rat IL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80329-ACR|
|Rat IL7 ORF mammalian expression plasmid, C-Flag tag||RG80329-CF|
|Rat IL7 ORF mammalian expression plasmid, C-His tag||RG80329-CH|
|Rat IL7 ORF mammalian expression plasmid, C-Myc tag||RG80329-CM|
|Rat IL7 ORF mammalian expression plasmid, C-HA tag||RG80329-CY|
|Rat IL7 Gene cDNA clone plasmid||RG80329-G|
|Rat IL7 ORF mammalian expression plasmid, N-Flag tag||RG80329-NF|
|Rat IL7 ORF mammalian expression plasmid, N-His tag||RG80329-NH|
|Rat IL7 ORF mammalian expression plasmid, N-Myc tag||RG80329-NM|
|Rat IL7 ORF mammalian expression plasmid, N-HA tag||RG80329-NY|
|Rat IL7 natural ORF mammalian expression plasmid||RG80329-UT|
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IL7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.