|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MAP2K4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50643-ACG|
|Mouse MAP2K4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50643-ACR|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-GFPSpark tag||MG50643-ANG|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50643-ANR|
|Mouse MAP2K4 ORF mammalian expression plasmid, C-Flag tag||MG50643-CF|
|Mouse MAP2K4 ORF mammalian expression plasmid, C-His tag||MG50643-CH|
|Mouse MAP2K4 ORF mammalian expression plasmid, C-Myc tag||MG50643-CM|
|Mouse MAP2K4 ORF mammalian expression plasmid, C-HA tag||MG50643-CY|
|Mouse MAP2K4 Gene cDNA clone plasmid||MG50643-M|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-Flag tag||MG50643-NF|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-His tag||MG50643-NH|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-Myc tag||MG50643-NM|
|Mouse MAP2K4 ORF mammalian expression plasmid, N-HA tag||MG50643-NY|
|Mouse MAP2K4 natural ORF mammalian expression plasmid||MG50643-UT|
|Learn more about expression Vectors|
Mouse dual specificity mitogen-activated protein kinase kinase 4, also known as MAP kinase kinase 4, MAPKK4, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, JNKK and MAP2K4, is a protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase subfamily. MAP2K4 / JNKK1 is a protein kinase which is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MAP2K4 / JNKK1 has been shown to activate MAPK8 / JNK1, MAPK9 / JNK2, and MAPK14 / p38, but not MAPK1 / ERK2 or MAPK3 / ERK1. MAP2K4 / JNKK1 is phosphorylated, and thus activated by MAP3K1 / MEKK. The stress-activated protein kinase (SAPK) pathways represent phosphorylation cascades that convey pro-apoptotic signals. The mitogen-activated protein kinase kinase (MAPKK) homolog MAP2K4 ( MKK4, SEK, JNKK1 ) is a centrally-placed mediator of the SAPK pathways.