|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat KIT ORF mammalian expression plasmid, C-GFPSpark tag||RG80321-ACG|
|Rat KIT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80321-ACR|
|Rat KIT ORF mammalian expression plasmid, C-Flag tag||RG80321-CF|
|Rat KIT ORF mammalian expression plasmid, C-His tag||RG80321-CH|
|Rat KIT ORF mammalian expression plasmid, C-Myc tag||RG80321-CM|
|Rat KIT ORF mammalian expression plasmid, C-HA tag||RG80321-CY|
|Rat KIT Gene cDNA clone plasmid||RG80321-G|
|Rat KIT ORF mammalian expression plasmid, N-Flag tag||RG80321-NF|
|Rat KIT ORF mammalian expression plasmid, N-His tag||RG80321-NH|
|Rat KIT ORF mammalian expression plasmid, N-Myc tag||RG80321-NM|
|Rat KIT ORF mammalian expression plasmid, N-HA tag||RG80321-NY|
|Rat KIT natural ORF mammalian expression plasmid||RG80321-UT|
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C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.