|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CNTN3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80316-ACG|
|Rat CNTN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80316-ACR|
|Rat CNTN3 ORF mammalian expression plasmid, C-Flag tag||RG80316-CF|
|Rat CNTN3 ORF mammalian expression plasmid, C-His tag||RG80316-CH|
|Rat CNTN3 ORF mammalian expression plasmid, C-Myc tag||RG80316-CM|
|Rat CNTN3 ORF mammalian expression plasmid, C-HA tag||RG80316-CY|
|Rat CNTN3 Gene cDNA clone plasmid||RG80316-G|
|Rat CNTN3 ORF mammalian expression plasmid, N-Flag tag||RG80316-NF|
|Rat CNTN3 ORF mammalian expression plasmid, N-His tag||RG80316-NH|
|Rat CNTN3 ORF mammalian expression plasmid, N-Myc tag||RG80316-NM|
|Rat CNTN3 ORF mammalian expression plasmid, N-HA tag||RG80316-NY|
|Rat CNTN3 natural ORF mammalian expression plasmid||RG80316-UT|
|Learn more about expression Vectors|
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.