|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat EPCAM ORF mammalian expression plasmid, C-GFPSpark tag||RG80306-ACG|
|Rat EPCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80306-ACR|
|Rat EPCAM ORF mammalian expression plasmid, C-Flag tag||RG80306-CF|
|Rat EPCAM ORF mammalian expression plasmid, C-His tag||RG80306-CH|
|Rat EPCAM ORF mammalian expression plasmid, C-Myc tag||RG80306-CM|
|Rat EPCAM ORF mammalian expression plasmid, C-HA tag||RG80306-CY|
|Rat EPCAM Gene cDNA clone plasmid||RG80306-G|
|Rat EPCAM ORF mammalian expression plasmid, N-Flag tag||RG80306-NF|
|Rat EPCAM ORF mammalian expression plasmid, N-His tag||RG80306-NH|
|Rat EPCAM ORF mammalian expression plasmid, N-Myc tag||RG80306-NM|
|Rat EPCAM ORF mammalian expression plasmid, N-HA tag||RG80306-NY|
|Rat EPCAM natural ORF mammalian expression plasmid||RG80306-UT|
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Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.