|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD53 ORF mammalian expression plasmid, C-GFPSpark tag||RG80303-ACG|
|Rat CD53 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80303-ACR|
|Rat CD53 ORF mammalian expression plasmid, C-Flag tag||RG80303-CF|
|Rat CD53 ORF mammalian expression plasmid, C-His tag||RG80303-CH|
|Rat CD53 ORF mammalian expression plasmid, C-Myc tag||RG80303-CM|
|Rat CD53 ORF mammalian expression plasmid, C-HA tag||RG80303-CY|
|Rat CD53 Gene cDNA clone plasmid||RG80303-G|
|Rat CD53 ORF mammalian expression plasmid, N-Flag tag||RG80303-NF|
|Rat CD53 ORF mammalian expression plasmid, N-His tag||RG80303-NH|
|Rat CD53 ORF mammalian expression plasmid, N-Myc tag||RG80303-NM|
|Rat CD53 ORF mammalian expression plasmid, N-HA tag||RG80303-NY|
|Rat CD53 natural ORF mammalian expression plasmid||RG80303-UT|
|Learn more about expression Vectors|
CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.