|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD59 ORF mammalian expression plasmid, C-GFPSpark tag||RG80299-ACG|
|Rat CD59 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80299-ACR|
|Rat CD59 ORF mammalian expression plasmid, C-Flag tag||RG80299-CF|
|Rat CD59 ORF mammalian expression plasmid, C-His tag||RG80299-CH|
|Rat CD59 ORF mammalian expression plasmid, C-Myc tag||RG80299-CM|
|Rat CD59 ORF mammalian expression plasmid, C-HA tag||RG80299-CY|
|Rat CD59 Gene cDNA clone plasmid||RG80299-G|
|Rat CD59 ORF mammalian expression plasmid, N-Flag tag||RG80299-NF|
|Rat CD59 ORF mammalian expression plasmid, N-His tag||RG80299-NH|
|Rat CD59 ORF mammalian expression plasmid, N-Myc tag||RG80299-NM|
|Rat CD59 ORF mammalian expression plasmid, N-HA tag||RG80299-NY|
|Rat CD59 natural ORF mammalian expression plasmid||RG80299-UT|
|Learn more about expression Vectors|
CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).