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Cynomolgus CD80 / B7-1 Protein (Fc Tag)

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Cynomolgus B7-1/CD80 Protein Product Information
Protein Construction:A DNA sequence encoding the cynomolgus CD80 (AAC31555.1) (Met1-Asn242) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Cynomolgus B7-1/CD80 Protein QC Testing
Purity:(89.4+9.8) % as determined by SDS-PAGE
Bio-Activity:Immobilized Cynomolgus CTLA4-His (Cat:90213-C08H) at 10 μg/ml (100 μl/well) can bind Cynomolgus CD80-Fc, The EC50 of CynomolgusCD80-Fc is 0.02-0.04 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Val 35
Molecule Mass:The recombinant cynomolgus CD80 is a disulfide-linked homodimer. The reduced monomer comprises 449 amino acids and has a calculated molecular mass of 50.9 KDa.The apparent molecular mass of the protein is approximately 65 and 44 KDa respectively in SDS-PAGE.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Cynomolgus B7-1/CD80 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other B7-1/CD80 Recombinant Protein Products
CD80/B7-1 Background

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Cynomolgus CD80/B7-1 References
  • Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.
  • Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.
  • Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
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    Catalog: 90268-C02H-100
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