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Cynomolgus B7-DC / PD-L2 / CD273 Protein (His Tag)

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Cynomolgus PD-L2 Protein Product Information
Protein Construction:A DNA sequence encoding the cynomolgus PDCD1LG2 (A4GW30) (Met1-Pro219) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Cynomolgus PD-L2 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA.
Immobilized Cynomolgus PDCD1LG2-His (Cat:90249-C08H) at 10 μg/ml (100 μl/well) can bind human PD1-Fc (Cat:10377-H03H), The EC50 of human PD1-Fch (Cat:10377-H03H) is 0.09-0.21 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Leu 20
Molecule Mass:The recombinant cynomolgus PDCD1LG2 comprises 211 amino acids and has a calculated molecular mass of 24 KDa. The apparent molecular mass of it is approximately 37-43 KDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Cynomolgus PD-L2 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cynomolgus PD-L2 Protein SDS-PAGE
Cynomolgus B7-DC / PD-L2 / CD273 Protein (His Tag) SDS-PAGE
Other PD-L2 Recombinant Protein Products
B7-DC/PD-L2/CD273 Background

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-&gamma; in monocytes, or LPS plus IFN-&gamma; in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Cynomolgus B7-DC/PD-L2/CD273 References
  • Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
  • Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
  • Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.
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    Size / Price
    Catalog: 90249-C08H-100
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    AvailabilityIn StockShipping instructions
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.