|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse EPHB4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50582-ACG|
|Mouse EPHB4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50582-ACR|
|Mouse EPHB4 ORF mammalian expression plasmid, C-Flag tag||MG50582-CF|
|Mouse EPHB4 ORF mammalian expression plasmid, C-His tag||MG50582-CH|
|Mouse EPHB4 ORF mammalian expression plasmid, C-Myc tag||MG50582-CM|
|Mouse EPHB4 ORF mammalian expression plasmid, C-HA tag||MG50582-CY|
|Mouse EPHB4 Gene cDNA clone plasmid||MG50582-M|
|Mouse EPHB4 ORF mammalian expression plasmid, N-Flag tag||MG50582-NF|
|Mouse EPHB4 ORF mammalian expression plasmid, N-His tag||MG50582-NH|
|Mouse EPHB4 ORF mammalian expression plasmid, N-Myc tag||MG50582-NM|
|Mouse EPHB4 ORF mammalian expression plasmid, N-HA tag||MG50582-NY|
|Mouse EPHB4 natural ORF mammalian expression plasmid||MG50582-UT|
|Learn more about expression Vectors|
Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.