|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD48 ORF mammalian expression plasmid, C-GFPSpark tag||RG80286-ACG|
|Rat CD48 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80286-ACR|
|Rat CD48 ORF mammalian expression plasmid, C-Flag tag||RG80286-CF|
|Rat CD48 ORF mammalian expression plasmid, C-His tag||RG80286-CH|
|Rat CD48 ORF mammalian expression plasmid, C-Myc tag||RG80286-CM|
|Rat CD48 ORF mammalian expression plasmid, C-HA tag||RG80286-CY|
|Rat CD48 Gene cDNA clone plasmid||RG80286-G|
|Rat CD48 ORF mammalian expression plasmid, N-Flag tag||RG80286-NF|
|Rat CD48 ORF mammalian expression plasmid, N-His tag||RG80286-NH|
|Rat CD48 ORF mammalian expression plasmid, N-Myc tag||RG80286-NM|
|Rat CD48 ORF mammalian expression plasmid, N-HA tag||RG80286-NY|
|Rat CD48 natural ORF mammalian expression plasmid||RG80286-UT|
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Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.