|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse SLAMF8 ORF mammalian expression plasmid, C-GFPSpark tag||MG50623-ACG|
|Mouse SLAMF8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50623-ACR|
|Mouse SLAMF8 ORF mammalian expression plasmid, C-Flag tag||MG50623-CF|
|Mouse SLAMF8 ORF mammalian expression plasmid, C-His tag||MG50623-CH|
|Mouse SLAMF8 ORF mammalian expression plasmid, C-Myc tag||MG50623-CM|
|Mouse SLAMF8 ORF mammalian expression plasmid, C-HA tag||MG50623-CY|
|Mouse SLAMF8 Gene cDNA clone plasmid||MG50623-M|
|Mouse SLAMF8 ORF mammalian expression plasmid, N-Flag tag||MG50623-NF|
|Mouse SLAMF8 ORF mammalian expression plasmid, N-His tag||MG50623-NH|
|Mouse SLAMF8 ORF mammalian expression plasmid, N-Myc tag||MG50623-NM|
|Mouse SLAMF8 ORF mammalian expression plasmid, N-HA tag||MG50623-NY|
|Mouse SLAMF8 natural ORF mammalian expression plasmid||MG50623-UT|
|Learn more about expression Vectors|
The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive and innate immune responses. These receptors share a common ectodomain organization: a membrane-proximal immunoglobulin constant domain and a membrane-distal immunoglobulin variable domain that is responsible for ligand recognition. SLAM family of receptors is expressed by a wide range of immune cells. Through their cytoplasmic domain, SLAM family receptors associate with SLAM-associated protein (SAP)-related molecules, a group of cytoplasmic adaptors composed almost exclusively of an SRC homology 2 domain. SLAM family receptors, in association with SAP family adaptors, have crucial roles during normal immune reactions in innate and adaptive immune cells.
Mouse SLAM family member 8, also known as B-lymphocyte activator macrophage expressed, BCM-like membrane protein, SLAMF8 and BLAME, is a single-pass type I membrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain. SLAMF8 / BLAME is expressed in lymph node, spleen, thymus and bone marrow. It may play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.