|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CDH13 ORF mammalian expression plasmid, C-GFPSpark tag||RG80280-ACG|
|Rat CDH13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80280-ACR|
|Rat CDH13 ORF mammalian expression plasmid, C-Flag tag||RG80280-CF|
|Rat CDH13 ORF mammalian expression plasmid, C-His tag||RG80280-CH|
|Rat CDH13 ORF mammalian expression plasmid, C-Myc tag||RG80280-CM|
|Rat CDH13 ORF mammalian expression plasmid, C-HA tag||RG80280-CY|
|Rat CDH13 Gene cDNA clone plasmid||RG80280-G|
|Rat CDH13 ORF mammalian expression plasmid, N-Flag tag||RG80280-NF|
|Rat CDH13 ORF mammalian expression plasmid, N-His tag||RG80280-NH|
|Rat CDH13 ORF mammalian expression plasmid, N-Myc tag||RG80280-NM|
|Rat CDH13 ORF mammalian expression plasmid, N-HA tag||RG80280-NY|
|Rat CDH13 natural ORF mammalian expression plasmid||RG80280-UT|
|Learn more about expression Vectors|
CDH13, also known as cadherin-13 and H Cadherin, is a member of the cadherin superfamily. CDH13 acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. CDH13 is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. CDH13 gene is hypermethylated in many types of cancer.