|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CDH5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80276-ACG|
|Rat CDH5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80276-ACR|
|Rat CDH5 ORF mammalian expression plasmid, C-Flag tag||RG80276-CF|
|Rat CDH5 ORF mammalian expression plasmid, C-His tag||RG80276-CH|
|Rat CDH5 ORF mammalian expression plasmid, C-Myc tag||RG80276-CM|
|Rat CDH5 ORF mammalian expression plasmid, C-HA tag||RG80276-CY|
|Rat CDH5 Gene cDNA clone plasmid||RG80276-G|
|Rat CDH5 ORF mammalian expression plasmid, N-Flag tag||RG80276-NF|
|Rat CDH5 ORF mammalian expression plasmid, N-His tag||RG80276-NH|
|Rat CDH5 ORF mammalian expression plasmid, N-Myc tag||RG80276-NM|
|Rat CDH5 ORF mammalian expression plasmid, N-HA tag||RG80276-NY|
|Rat CDH5 natural ORF mammalian expression plasmid||RG80276-UT|
|Learn more about expression Vectors|
Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based diagnoses, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.