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Cynomolgus CD62L / L-Selectin / SELL Protein (His Tag)

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Cynomolgus CD62L/SELL Protein Product Information
Protein Construction:A DNA sequence encoding the cynomolgus SELL (Met1-Asn332) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Cynomolgus CD62L/SELL Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 10E4 cells/well are added to cyno L-Selectin coated plates (10 μg/mL with 100 μL/well), > 60% cells will adhere for 1 hour incubation at 37℃.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Asp 29
Molecule Mass:The recombinant cynomolgus SELL comprises 315 amino acids and has a calculated molecular mass of 35.6 KDa. The apparent molecular mass of it is approximately 52-63 KDa respectively in SDS-PAGE.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Cynomolgus CD62L/SELL Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Cynomolgus CD62L/SELL Protein SDS-PAGE
Cynomolgus CD62L / L-Selectin / SELL Protein (His Tag) SDS-PAGE
Cynomolgus CD62L / L-Selectin / SELL Protein (His Tag) SDS-PAGE
Other CD62L/SELL Recombinant Protein Products
CD62L/L-Selectin Background

L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naïve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.

Cynomolgus CD62L/L-Selectin References
  • Smalley DM, et al. (2005) L-selectin: mechanisms and physiological significance of ectodomain cleavage. J Cell Mol Med. 9(2): 255-66.
  • Grailer JJ, et al. (2009) L-selectin: role in regulating homeostasis and cutaneous inflammation. J Dermatol Sci. 56(3): 141-7.
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    Catalog: 90168-C08H-200
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