|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat SIRPA ORF mammalian expression plasmid, C-GFPSpark tag||RG80270-ACG|
|Rat SIRPA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80270-ACR|
|Rat SIRPA ORF mammalian expression plasmid, C-Flag tag||RG80270-CF|
|Rat SIRPA ORF mammalian expression plasmid, C-His tag||RG80270-CH|
|Rat SIRPA ORF mammalian expression plasmid, C-Myc tag||RG80270-CM|
|Rat SIRPA ORF mammalian expression plasmid, C-HA tag||RG80270-CY|
|Rat SIRPA Gene cDNA clone plasmid||RG80270-G|
|Rat SIRPA ORF mammalian expression plasmid, N-Flag tag||RG80270-NF|
|Rat SIRPA ORF mammalian expression plasmid, N-His tag||RG80270-NH|
|Rat SIRPA ORF mammalian expression plasmid, N-Myc tag||RG80270-NM|
|Rat SIRPA ORF mammalian expression plasmid, N-HA tag||RG80270-NY|
|Rat SIRPA natural ORF mammalian expression plasmid||RG80270-UT|
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Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.