|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat DSC2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80266-ACG|
|Rat DSC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80266-ACR|
|Rat DSC2 ORF mammalian expression plasmid, C-Flag tag||RG80266-CF|
|Rat DSC2 ORF mammalian expression plasmid, C-His tag||RG80266-CH|
|Rat DSC2 ORF mammalian expression plasmid, C-Myc tag||RG80266-CM|
|Rat DSC2 ORF mammalian expression plasmid, C-HA tag||RG80266-CY|
|Rat DSC2 Gene cDNA clone plasmid||RG80266-G|
|Rat DSC2 ORF mammalian expression plasmid, N-Flag tag||RG80266-NF|
|Rat DSC2 ORF mammalian expression plasmid, N-His tag||RG80266-NH|
|Rat DSC2 ORF mammalian expression plasmid, N-Myc tag||RG80266-NM|
|Rat DSC2 ORF mammalian expression plasmid, N-HA tag||RG80266-NY|
|Rat DSC2 natural ORF mammalian expression plasmid||RG80266-UT|
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DSC2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in DSC2 are associated with arrhythmogenic right ventricular dysplasia-11. DSC2 is Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSC2 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms.