|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD34 ORF mammalian expression plasmid, C-GFPSpark tag||RG80262-ACG|
|Rat CD34 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80262-ACR|
|Rat CD34 ORF mammalian expression plasmid, C-Flag tag||RG80262-CF|
|Rat CD34 ORF mammalian expression plasmid, C-His tag||RG80262-CH|
|Rat CD34 ORF mammalian expression plasmid, C-Myc tag||RG80262-CM|
|Rat CD34 ORF mammalian expression plasmid, C-HA tag||RG80262-CY|
|Rat CD34 Gene cDNA clone plasmid||RG80262-G|
|Rat CD34 ORF mammalian expression plasmid, N-Flag tag||RG80262-NF|
|Rat CD34 ORF mammalian expression plasmid, N-His tag||RG80262-NH|
|Rat CD34 ORF mammalian expression plasmid, N-Myc tag||RG80262-NM|
|Rat CD34 ORF mammalian expression plasmid, N-HA tag||RG80262-NY|
|Rat CD34 natural ORF mammalian expression plasmid||RG80262-UT|
|Learn more about expression Vectors|
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.