|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse ARMETL1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50569-ACG|
|Mouse ARMETL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50569-ACR|
|Mouse ARMETL1 ORF mammalian expression plasmid, C-Flag tag||MG50569-CF|
|Mouse ARMETL1 ORF mammalian expression plasmid, C-His tag||MG50569-CH|
|Mouse ARMETL1 ORF mammalian expression plasmid, C-Myc tag||MG50569-CM|
|Mouse ARMETL1 ORF mammalian expression plasmid, C-HA tag||MG50569-CY|
|Mouse ARMETL1 Gene cDNA clone plasmid||MG50569-M|
|Mouse ARMETL1 ORF mammalian expression plasmid, N-Flag tag||MG50569-NF|
|Mouse ARMETL1 ORF mammalian expression plasmid, N-His tag||MG50569-NH|
|Mouse ARMETL1 ORF mammalian expression plasmid, N-Myc tag||MG50569-NM|
|Mouse ARMETL1 ORF mammalian expression plasmid, N-HA tag||MG50569-NY|
|Mouse ARMETL1 natural ORF mammalian expression plasmid||MG50569-UT|
|Learn more about expression Vectors|
Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.