|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat KLRK1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80250-ACG|
|Rat KLRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80250-ACR|
|Rat KLRK1 ORF mammalian expression plasmid, C-Flag tag||RG80250-CF|
|Rat KLRK1 ORF mammalian expression plasmid, C-His tag||RG80250-CH|
|Rat KLRK1 ORF mammalian expression plasmid, C-Myc tag||RG80250-CM|
|Rat KLRK1 ORF mammalian expression plasmid, C-HA tag||RG80250-CY|
|Rat KLRK1 Gene cDNA clone plasmid||RG80250-G|
|Rat KLRK1 ORF mammalian expression plasmid, N-Flag tag||RG80250-NF|
|Rat KLRK1 ORF mammalian expression plasmid, N-His tag||RG80250-NH|
|Rat KLRK1 ORF mammalian expression plasmid, N-Myc tag||RG80250-NM|
|Rat KLRK1 ORF mammalian expression plasmid, N-HA tag||RG80250-NY|
|Rat KLRK1 natural ORF mammalian expression plasmid||RG80250-UT|
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NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.