|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat LGALS1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80248-ACG|
|Rat LGALS1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80248-ACR|
|Rat LGALS1 ORF mammalian expression plasmid, C-Flag tag||RG80248-CF|
|Rat LGALS1 ORF mammalian expression plasmid, C-His tag||RG80248-CH|
|Rat LGALS1 ORF mammalian expression plasmid, C-Myc tag||RG80248-CM|
|Rat LGALS1 ORF mammalian expression plasmid, C-HA tag||RG80248-CY|
|Rat LGALS1 Gene cDNA clone plasmid||RG80248-G|
|Rat LGALS1 ORF mammalian expression plasmid, N-Flag tag||RG80248-NF|
|Rat LGALS1 ORF mammalian expression plasmid, N-His tag||RG80248-NH|
|Rat LGALS1 ORF mammalian expression plasmid, N-Myc tag||RG80248-NM|
|Rat LGALS1 ORF mammalian expression plasmid, N-HA tag||RG80248-NY|
|Rat LGALS1 natural ORF mammalian expression plasmid||RG80248-UT|
|Learn more about expression Vectors|
Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.