|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse RSPO3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50606-ACG|
|Mouse RSPO3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50606-ACR|
|Mouse RSPO3 ORF mammalian expression plasmid, C-Flag tag||MG50606-CF|
|Mouse RSPO3 ORF mammalian expression plasmid, C-His tag||MG50606-CH|
|Mouse RSPO3 ORF mammalian expression plasmid, C-Myc tag||MG50606-CM|
|Mouse RSPO3 ORF mammalian expression plasmid, C-HA tag||MG50606-CY|
|Mouse RSPO3 Gene cDNA clone plasmid||MG50606-M|
|Mouse RSPO3 ORF mammalian expression plasmid, N-Flag tag||MG50606-NF|
|Mouse RSPO3 ORF mammalian expression plasmid, N-His tag||MG50606-NH|
|Mouse RSPO3 ORF mammalian expression plasmid, N-Myc tag||MG50606-NM|
|Mouse RSPO3 ORF mammalian expression plasmid, N-HA tag||MG50606-NY|
|Mouse RSPO3 natural ORF mammalian expression plasmid||MG50606-UT|
|Learn more about expression Vectors|
R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.