|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ICAM2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80239-ACG|
|Rat ICAM2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80239-ACR|
|Rat ICAM2 ORF mammalian expression plasmid, C-Flag tag||RG80239-CF|
|Rat ICAM2 ORF mammalian expression plasmid, C-His tag||RG80239-CH|
|Rat ICAM2 ORF mammalian expression plasmid, C-Myc tag||RG80239-CM|
|Rat ICAM2 ORF mammalian expression plasmid, C-HA tag||RG80239-CY|
|Rat ICAM2 Gene cDNA clone plasmid||RG80239-G|
|Rat ICAM2 ORF mammalian expression plasmid, N-Flag tag||RG80239-NF|
|Rat ICAM2 ORF mammalian expression plasmid, N-His tag||RG80239-NH|
|Rat ICAM2 ORF mammalian expression plasmid, N-Myc tag||RG80239-NM|
|Rat ICAM2 ORF mammalian expression plasmid, N-HA tag||RG80239-NY|
|Rat ICAM2 natural ORF mammalian expression plasmid||RG80239-UT|
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Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.