|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat MDH1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80232-ACG|
|Rat MDH1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80232-ACR|
|Rat MDH1 ORF mammalian expression plasmid, N-GFPSpark tag||RG80232-ANG|
|Rat MDH1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80232-ANR|
|Rat MDH1 ORF mammalian expression plasmid, C-Flag tag||RG80232-CF|
|Rat MDH1 ORF mammalian expression plasmid, C-His tag||RG80232-CH|
|Rat MDH1 ORF mammalian expression plasmid, C-Myc tag||RG80232-CM|
|Rat MDH1 ORF mammalian expression plasmid, C-HA tag||RG80232-CY|
|Rat MDH1 Gene cDNA clone plasmid||RG80232-G|
|Rat MDH1 ORF mammalian expression plasmid, N-Flag tag||RG80232-NF|
|Rat MDH1 ORF mammalian expression plasmid, N-His tag||RG80232-NH|
|Rat MDH1 ORF mammalian expression plasmid, N-Myc tag||RG80232-NM|
|Rat MDH1 ORF mammalian expression plasmid, N-HA tag||RG80232-NY|
|Rat MDH1 natural ORF mammalian expression plasmid||RG80232-UT|
|Learn more about expression Vectors|
Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.