|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat BCAM ORF mammalian expression plasmid, C-GFPSpark tag||RG80222-ACG|
|Rat BCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80222-ACR|
|Rat BCAM ORF mammalian expression plasmid, C-Flag tag||RG80222-CF|
|Rat BCAM ORF mammalian expression plasmid, C-His tag||RG80222-CH|
|Rat BCAM ORF mammalian expression plasmid, C-Myc tag||RG80222-CM|
|Rat BCAM ORF mammalian expression plasmid, C-HA tag||RG80222-CY|
|Rat BCAM Gene cDNA clone plasmid||RG80222-G|
|Rat BCAM ORF mammalian expression plasmid, N-Flag tag||RG80222-NF|
|Rat BCAM ORF mammalian expression plasmid, N-His tag||RG80222-NH|
|Rat BCAM ORF mammalian expression plasmid, N-Myc tag||RG80222-NM|
|Rat BCAM ORF mammalian expression plasmid, N-HA tag||RG80222-NY|
|Rat BCAM natural ORF mammalian expression plasmid||RG80222-UT|
|Learn more about expression Vectors|
The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.