|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TMEM27 ORF mammalian expression plasmid, C-GFPSpark tag||MG50547-ACG|
|Mouse TMEM27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50547-ACR|
|Mouse TMEM27 ORF mammalian expression plasmid, C-Flag tag||MG50547-CF|
|Mouse TMEM27 ORF mammalian expression plasmid, C-His tag||MG50547-CH|
|Mouse TMEM27 ORF mammalian expression plasmid, C-Myc tag||MG50547-CM|
|Mouse TMEM27 ORF mammalian expression plasmid, C-HA tag||MG50547-CY|
|Mouse TMEM27 Gene cDNA clone plasmid||MG50547-M|
|Mouse TMEM27 ORF mammalian expression plasmid, N-Flag tag||MG50547-NF|
|Mouse TMEM27 ORF mammalian expression plasmid, N-His tag||MG50547-NH|
|Mouse TMEM27 ORF mammalian expression plasmid, N-Myc tag||MG50547-NM|
|Mouse TMEM27 ORF mammalian expression plasmid, N-HA tag||MG50547-NY|
|Mouse TMEM27 natural ORF mammalian expression plasmid||MG50547-UT|
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TMEM27 is a membrane protein. It has been proposed as a beta cell mass biomarker since it is cleaved and shed by pancreatic beta cells. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is also important for trafficking amino acid transporters to the apical brush border of proximal tubules.