|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse FLT4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50584-ACG|
|Mouse FLT4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50584-ACR|
|Mouse FLT4 ORF mammalian expression plasmid, C-Flag tag||MG50584-CF|
|Mouse FLT4 ORF mammalian expression plasmid, C-His tag||MG50584-CH|
|Mouse FLT4 ORF mammalian expression plasmid, C-Myc tag||MG50584-CM|
|Mouse FLT4 ORF mammalian expression plasmid, C-HA tag||MG50584-CY|
|Mouse FLT4 Gene cDNA clone plasmid||MG50584-M|
|Mouse FLT4 ORF mammalian expression plasmid, N-Flag tag||MG50584-NF|
|Mouse FLT4 ORF mammalian expression plasmid, N-His tag||MG50584-NH|
|Mouse FLT4 ORF mammalian expression plasmid, N-Myc tag||MG50584-NM|
|Mouse FLT4 ORF mammalian expression plasmid, N-HA tag||MG50584-NY|
|Mouse FLT4 natural ORF mammalian expression plasmid||MG50584-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.