|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL27 ORF mammalian expression plasmid, C-GFPSpark tag||RG80201-ACG|
|Rat IL27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80201-ACR|
|Rat IL27 ORF mammalian expression plasmid, C-Flag tag||RG80201-CF|
|Rat IL27 ORF mammalian expression plasmid, C-His tag||RG80201-CH|
|Rat IL27 ORF mammalian expression plasmid, C-Myc tag||RG80201-CM|
|Rat IL27 ORF mammalian expression plasmid, C-HA tag||RG80201-CY|
|Rat IL27 Gene cDNA clone plasmid||RG80201-G|
|Rat IL27 ORF mammalian expression plasmid, N-Flag tag||RG80201-NF|
|Rat IL27 ORF mammalian expression plasmid, N-His tag||RG80201-NH|
|Rat IL27 ORF mammalian expression plasmid, N-Myc tag||RG80201-NM|
|Rat IL27 ORF mammalian expression plasmid, N-HA tag||RG80201-NY|
|Rat IL27 natural ORF mammalian expression plasmid||RG80201-UT|
|Learn more about expression Vectors|
IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. IL-27 protein is also referred as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.