|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TNFRSF18 ORF mammalian expression plasmid, C-GFPSpark tag||RG80192-ACG|
|Rat TNFRSF18 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80192-ACR|
|Rat TNFRSF18 ORF mammalian expression plasmid, C-Flag tag||RG80192-CF|
|Rat TNFRSF18 ORF mammalian expression plasmid, C-His tag||RG80192-CH|
|Rat TNFRSF18 ORF mammalian expression plasmid, C-Myc tag||RG80192-CM|
|Rat TNFRSF18 ORF mammalian expression plasmid, C-HA tag||RG80192-CY|
|Rat TNFRSF18 Gene cDNA clone plasmid||RG80192-G|
|Rat TNFRSF18 ORF mammalian expression plasmid, N-Flag tag||RG80192-NF|
|Rat TNFRSF18 ORF mammalian expression plasmid, N-His tag||RG80192-NH|
|Rat TNFRSF18 ORF mammalian expression plasmid, N-Myc tag||RG80192-NM|
|Rat TNFRSF18 ORF mammalian expression plasmid, N-HA tag||RG80192-NY|
|Rat TNFRSF18 natural ORF mammalian expression plasmid||RG80192-UT|
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GITR, also known as TNFRSF18(CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR plays a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway.