|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL12RB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80185-ACG|
|Rat IL12RB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80185-ACR|
|Rat IL12RB2 ORF mammalian expression plasmid, C-Flag tag||RG80185-CF|
|Rat IL12RB2 ORF mammalian expression plasmid, C-His tag||RG80185-CH|
|Rat IL12RB2 ORF mammalian expression plasmid, C-Myc tag||RG80185-CM|
|Rat IL12RB2 ORF mammalian expression plasmid, C-HA tag||RG80185-CY|
|Rat IL12RB2 Gene cDNA clone plasmid||RG80185-G|
|Rat IL12RB2 ORF mammalian expression plasmid, N-Flag tag||RG80185-NF|
|Rat IL12RB2 ORF mammalian expression plasmid, N-His tag||RG80185-NH|
|Rat IL12RB2 ORF mammalian expression plasmid, N-Myc tag||RG80185-NM|
|Rat IL12RB2 ORF mammalian expression plasmid, N-HA tag||RG80185-NY|
|Rat IL12RB2 natural ORF mammalian expression plasmid||RG80185-UT|
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Interleukin-12 receptor subunit beta-2 (IL12RB2), also known as IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, and IL-12RB2, is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. IL12RB2 belongs to the type I cytokine receptor family. The coexpression of IL12RB2 and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of IL12RB2 is up-regulated by IFN gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of IL12RB2 is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. IL12RB2 promotes the proliferation of T-cells as well as NK cells. IL12RB2 induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.