|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL10RB ORF mammalian expression plasmid, C-GFPSpark tag||RG80184-ACG|
|Rat IL10RB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80184-ACR|
|Rat IL10RB ORF mammalian expression plasmid, C-Flag tag||RG80184-CF|
|Rat IL10RB ORF mammalian expression plasmid, C-His tag||RG80184-CH|
|Rat IL10RB ORF mammalian expression plasmid, C-Myc tag||RG80184-CM|
|Rat IL10RB ORF mammalian expression plasmid, C-HA tag||RG80184-CY|
|Rat IL10RB Gene cDNA clone plasmid||RG80184-G|
|Rat IL10RB ORF mammalian expression plasmid, N-Flag tag||RG80184-NF|
|Rat IL10RB ORF mammalian expression plasmid, N-His tag||RG80184-NH|
|Rat IL10RB ORF mammalian expression plasmid, N-Myc tag||RG80184-NM|
|Rat IL10RB ORF mammalian expression plasmid, N-HA tag||RG80184-NY|
|Rat IL10RB natural ORF mammalian expression plasmid||RG80184-UT|
|Learn more about expression Vectors|
Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.