|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IFNAR1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80180-ACG|
|Rat IFNAR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80180-ACR|
|Rat IFNAR1 ORF mammalian expression plasmid, C-Flag tag||RG80180-CF|
|Rat IFNAR1 ORF mammalian expression plasmid, C-His tag||RG80180-CH|
|Rat IFNAR1 ORF mammalian expression plasmid, C-Myc tag||RG80180-CM|
|Rat IFNAR1 ORF mammalian expression plasmid, C-HA tag||RG80180-CY|
|Rat IFNAR1 Gene cDNA clone plasmid||RG80180-G|
|Rat IFNAR1 ORF mammalian expression plasmid, N-Flag tag||RG80180-NF|
|Rat IFNAR1 ORF mammalian expression plasmid, N-His tag||RG80180-NH|
|Rat IFNAR1 ORF mammalian expression plasmid, N-Myc tag||RG80180-NM|
|Rat IFNAR1 ORF mammalian expression plasmid, N-HA tag||RG80180-NY|
|Rat IFNAR1 natural ORF mammalian expression plasmid||RG80180-UT|
|Learn more about expression Vectors|
Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.