|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse HPGD ORF mammalian expression plasmid, C-GFPSpark tag||MG50531-ACG|
|Mouse HPGD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50531-ACR|
|Mouse HPGD ORF mammalian expression plasmid, N-GFPSpark tag||MG50531-ANG|
|Mouse HPGD ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50531-ANR|
|Mouse HPGD ORF mammalian expression plasmid, C-Flag tag||MG50531-CF|
|Mouse HPGD ORF mammalian expression plasmid, C-His tag||MG50531-CH|
|Mouse HPGD ORF mammalian expression plasmid, C-Myc tag||MG50531-CM|
|Mouse HPGD ORF mammalian expression plasmid, C-HA tag||MG50531-CY|
|Mouse HPGD Gene cDNA clone plasmid||MG50531-M|
|Mouse HPGD ORF mammalian expression plasmid, N-Flag tag||MG50531-NF|
|Mouse HPGD ORF mammalian expression plasmid, N-His tag||MG50531-NH|
|Mouse HPGD ORF mammalian expression plasmid, N-Myc tag||MG50531-NM|
|Mouse HPGD ORF mammalian expression plasmid, N-HA tag||MG50531-NY|
|Mouse HPGD natural ORF mammalian expression plasmid||MG50531-UT|
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Mouse 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.