|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TNFRSF12A ORF mammalian expression plasmid, C-GFPSpark tag||RG80164-ACG|
|Rat TNFRSF12A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80164-ACR|
|Rat TNFRSF12A ORF mammalian expression plasmid, C-Flag tag||RG80164-CF|
|Rat TNFRSF12A ORF mammalian expression plasmid, C-His tag||RG80164-CH|
|Rat TNFRSF12A ORF mammalian expression plasmid, C-Myc tag||RG80164-CM|
|Rat TNFRSF12A ORF mammalian expression plasmid, C-HA tag||RG80164-CY|
|Rat TNFRSF12A Gene cDNA clone plasmid||RG80164-G|
|Rat TNFRSF12A ORF mammalian expression plasmid, N-Flag tag||RG80164-NF|
|Rat TNFRSF12A ORF mammalian expression plasmid, N-His tag||RG80164-NH|
|Rat TNFRSF12A ORF mammalian expression plasmid, N-Myc tag||RG80164-NM|
|Rat TNFRSF12A ORF mammalian expression plasmid, N-HA tag||RG80164-NY|
|Rat TNFRSF12A natural ORF mammalian expression plasmid||RG80164-UT|
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Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Fn14 shares 82% amino acid identity with the mouse sequence. It contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. In response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro, the level of Fn14 is increased. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.