|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CX3CL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80163-ACG|
|Rat CX3CL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80163-ACR|
|Rat CX3CL1 ORF mammalian expression plasmid, C-Flag tag||RG80163-CF|
|Rat CX3CL1 ORF mammalian expression plasmid, C-His tag||RG80163-CH|
|Rat CX3CL1 ORF mammalian expression plasmid, C-Myc tag||RG80163-CM|
|Rat CX3CL1 ORF mammalian expression plasmid, C-HA tag||RG80163-CY|
|Rat CX3CL1 Gene cDNA clone plasmid||RG80163-G|
|Rat CX3CL1 ORF mammalian expression plasmid, N-Flag tag||RG80163-NF|
|Rat CX3CL1 ORF mammalian expression plasmid, N-His tag||RG80163-NH|
|Rat CX3CL1 ORF mammalian expression plasmid, N-Myc tag||RG80163-NM|
|Rat CX3CL1 ORF mammalian expression plasmid, N-HA tag||RG80163-NY|
|Rat CX3CL1 natural ORF mammalian expression plasmid||RG80163-UT|
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Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.