|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat TNFSF12 ORF mammalian expression plasmid, C-GFPSpark tag||RG80154-ACG|
|Rat TNFSF12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80154-ACR|
|Rat TNFSF12 ORF mammalian expression plasmid, C-Flag tag||RG80154-CF|
|Rat TNFSF12 ORF mammalian expression plasmid, C-His tag||RG80154-CH|
|Rat TNFSF12 ORF mammalian expression plasmid, C-Myc tag||RG80154-CM|
|Rat TNFSF12 ORF mammalian expression plasmid, C-HA tag||RG80154-CY|
|Rat TNFSF12 Gene cDNA clone plasmid||RG80154-G|
|Rat TNFSF12 ORF mammalian expression plasmid, N-Flag tag||RG80154-NF|
|Rat TNFSF12 ORF mammalian expression plasmid, N-His tag||RG80154-NH|
|Rat TNFSF12 ORF mammalian expression plasmid, N-Myc tag||RG80154-NM|
|Rat TNFSF12 ORF mammalian expression plasmid, N-HA tag||RG80154-NY|
|Rat TNFSF12 natural ORF mammalian expression plasmid||RG80154-UT|
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TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.