|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CES2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50515-ACG|
|Mouse CES2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50515-ACR|
|Mouse CES2 ORF mammalian expression plasmid, C-Flag tag||MG50515-CF|
|Mouse CES2 ORF mammalian expression plasmid, C-His tag||MG50515-CH|
|Mouse CES2 ORF mammalian expression plasmid, C-Myc tag||MG50515-CM|
|Mouse CES2 ORF mammalian expression plasmid, C-HA tag||MG50515-CY|
|Mouse CES2 Gene cDNA clone plasmid||MG50515-M|
|Mouse CES2 ORF mammalian expression plasmid, N-Flag tag||MG50515-NF|
|Mouse CES2 ORF mammalian expression plasmid, N-His tag||MG50515-NH|
|Mouse CES2 ORF mammalian expression plasmid, N-Myc tag||MG50515-NM|
|Mouse CES2 ORF mammalian expression plasmid, N-HA tag||MG50515-NY|
|Mouse CES2 natural ORF mammalian expression plasmid||MG50515-UT|
|Learn more about expression Vectors|
Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.