|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse MERTK ORF mammalian expression plasmid, C-GFPSpark tag||MG50514-ACG|
|Mouse MERTK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50514-ACR|
|Mouse MERTK ORF mammalian expression plasmid, C-Flag tag||MG50514-CF|
|Mouse MERTK ORF mammalian expression plasmid, C-His tag||MG50514-CH|
|Mouse MERTK ORF mammalian expression plasmid, C-Myc tag||MG50514-CM|
|Mouse MERTK ORF mammalian expression plasmid, C-HA tag||MG50514-CY|
|Mouse MERTK Gene cDNA clone plasmid||MG50514-M|
|Mouse MERTK ORF mammalian expression plasmid, N-Flag tag||MG50514-NF|
|Mouse MERTK ORF mammalian expression plasmid, N-His tag||MG50514-NH|
|Mouse MERTK ORF mammalian expression plasmid, N-Myc tag||MG50514-NM|
|Mouse MERTK ORF mammalian expression plasmid, N-HA tag||MG50514-NY|
|Mouse MERTK natural ORF mammalian expression plasmid||MG50514-UT|
|Learn more about expression Vectors|
&Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.