|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD40 ORF mammalian expression plasmid, C-GFPSpark tag||RG80151-ACG|
|Rat CD40 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80151-ACR|
|Rat CD40 ORF mammalian expression plasmid, C-Flag tag||RG80151-CF|
|Rat CD40 ORF mammalian expression plasmid, C-His tag||RG80151-CH|
|Rat CD40 ORF mammalian expression plasmid, C-Myc tag||RG80151-CM|
|Rat CD40 ORF mammalian expression plasmid, C-HA tag||RG80151-CY|
|Rat CD40 Gene cDNA clone plasmid||RG80151-G|
|Rat CD40 ORF mammalian expression plasmid, N-Flag tag||RG80151-NF|
|Rat CD40 ORF mammalian expression plasmid, N-His tag||RG80151-NH|
|Rat CD40 ORF mammalian expression plasmid, N-Myc tag||RG80151-NM|
|Rat CD40 ORF mammalian expression plasmid, N-HA tag||RG80151-NY|
|Rat CD40 natural ORF mammalian expression plasmid||RG80151-UT|
|Learn more about expression Vectors|
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.