|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ACVR1B ORF mammalian expression plasmid, C-GFPSpark tag||RG80137-ACG|
|Rat ACVR1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80137-ACR|
|Rat ACVR1B ORF mammalian expression plasmid, C-Flag tag||RG80137-CF|
|Rat ACVR1B ORF mammalian expression plasmid, C-His tag||RG80137-CH|
|Rat ACVR1B ORF mammalian expression plasmid, C-Myc tag||RG80137-CM|
|Rat ACVR1B ORF mammalian expression plasmid, C-HA tag||RG80137-CY|
|Rat ACVR1B Gene cDNA clone plasmid||RG80137-G|
|Rat ACVR1B ORF mammalian expression plasmid, N-Flag tag||RG80137-NF|
|Rat ACVR1B ORF mammalian expression plasmid, N-His tag||RG80137-NH|
|Rat ACVR1B ORF mammalian expression plasmid, N-Myc tag||RG80137-NM|
|Rat ACVR1B ORF mammalian expression plasmid, N-HA tag||RG80137-NY|
|Rat ACVR1B natural ORF mammalian expression plasmid||RG80137-UT|
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ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.