|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat ACVRL1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80132-ACG|
|Rat ACVRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80132-ACR|
|Rat ACVRL1 ORF mammalian expression plasmid, C-Flag tag||RG80132-CF|
|Rat ACVRL1 ORF mammalian expression plasmid, C-His tag||RG80132-CH|
|Rat ACVRL1 ORF mammalian expression plasmid, C-Myc tag||RG80132-CM|
|Rat ACVRL1 ORF mammalian expression plasmid, C-HA tag||RG80132-CY|
|Rat ACVRL1 Gene cDNA clone plasmid||RG80132-G|
|Rat ACVRL1 ORF mammalian expression plasmid, N-Flag tag||RG80132-NF|
|Rat ACVRL1 ORF mammalian expression plasmid, N-His tag||RG80132-NH|
|Rat ACVRL1 ORF mammalian expression plasmid, N-Myc tag||RG80132-NM|
|Rat ACVRL1 ORF mammalian expression plasmid, N-HA tag||RG80132-NY|
|Rat ACVRL1 natural ORF mammalian expression plasmid||RG80132-UT|
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Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.