|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat BMPR1B ORF mammalian expression plasmid, C-GFPSpark tag||RG80128-ACG|
|Rat BMPR1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80128-ACR|
|Rat BMPR1B ORF mammalian expression plasmid, C-Flag tag||RG80128-CF|
|Rat BMPR1B ORF mammalian expression plasmid, C-His tag||RG80128-CH|
|Rat BMPR1B ORF mammalian expression plasmid, C-Myc tag||RG80128-CM|
|Rat BMPR1B ORF mammalian expression plasmid, C-HA tag||RG80128-CY|
|Rat BMPR1B Gene cDNA clone plasmid||RG80128-G|
|Rat BMPR1B ORF mammalian expression plasmid, N-Flag tag||RG80128-NF|
|Rat BMPR1B ORF mammalian expression plasmid, N-His tag||RG80128-NH|
|Rat BMPR1B ORF mammalian expression plasmid, N-Myc tag||RG80128-NM|
|Rat BMPR1B ORF mammalian expression plasmid, N-HA tag||RG80128-NY|
|Rat BMPR1B natural ORF mammalian expression plasmid||RG80128-UT|
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BMPR1B(bone morphogenetic protein receptor, type IB), also known as ALK6, is a a member of the bone morphogenetic protein (BMP) receptor family. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. BMPR1B is the major transducer of signals in precartilaginous condensations as demonstrated in experiments using constitutively active BMPR1B receptors. BMPR1B is a more effective trasducer of GDF5 than BMPR1A. Unlike BMPR1A null mice, which die at an early embryonic stage, BMPR1B null mice are viable.