|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CTLA4 ORF mammalian expression plasmid, C-GFPSpark tag||MG50503-ACG|
|Mouse CTLA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50503-ACR|
|Mouse CTLA4 ORF mammalian expression plasmid, C-Flag tag||MG50503-CF|
|Mouse CTLA4 ORF mammalian expression plasmid, C-His tag||MG50503-CH|
|Mouse CTLA4 ORF mammalian expression plasmid, C-Myc tag||MG50503-CM|
|Mouse CTLA4 ORF mammalian expression plasmid, C-HA tag||MG50503-CY|
|Mouse CTLA4 Gene cDNA clone plasmid||MG50503-M|
|Mouse CTLA4 ORF mammalian expression plasmid, N-Flag tag||MG50503-NF|
|Mouse CTLA4 ORF mammalian expression plasmid, N-His tag||MG50503-NH|
|Mouse CTLA4 ORF mammalian expression plasmid, N-Myc tag||MG50503-NM|
|Mouse CTLA4 ORF mammalian expression plasmid, N-HA tag||MG50503-NY|
|Mouse CTLA4 natural ORF mammalian expression plasmid||MG50503-UT|
|Learn more about expression Vectors|
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).