|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse TGFBR3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50542-ACG|
|Mouse TGFBR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50542-ACR|
|Mouse TGFBR3 ORF mammalian expression plasmid, C-Flag tag||MG50542-CF|
|Mouse TGFBR3 ORF mammalian expression plasmid, C-His tag||MG50542-CH|
|Mouse TGFBR3 ORF mammalian expression plasmid, C-Myc tag||MG50542-CM|
|Mouse TGFBR3 ORF mammalian expression plasmid, C-HA tag||MG50542-CY|
|Mouse TGFBR3 Gene cDNA clone plasmid||MG50542-M|
|Mouse TGFBR3 ORF mammalian expression plasmid, N-Flag tag||MG50542-NF|
|Mouse TGFBR3 ORF mammalian expression plasmid, N-His tag||MG50542-NH|
|Mouse TGFBR3 ORF mammalian expression plasmid, N-Myc tag||MG50542-NM|
|Mouse TGFBR3 ORF mammalian expression plasmid, N-HA tag||MG50542-NY|
|Mouse TGFBR3 natural ORF mammalian expression plasmid||MG50542-UT|
|Learn more about expression Vectors|
Betaglycan also known as transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate / heparan sulfate proteoglycan. TGFBR3 is a transforming growth factor (TGF)-beta type III receptor. This receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is the TGF-β component most commonly downregulated among localized human prostate cancer studies. TGFBR3 knockdown led to focus formation and enhanced expression of CD133, a marker found on prostate cancer stem cells. TGFBR3 is an accessory receptor that binds to and modulates the activities of both transforming growth factor-beta (TGFβ) and inhibin, two members of the TGFβ superfamily of growth factors that regulate many aspects of reproductive biology. TGFBR3 is known to be expressed in adult testis and ovary, but little is known about this receptor during gonadogenesis.