|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CFD ORF mammalian expression plasmid, C-GFPSpark tag||MG50539-ACG|
|Mouse CFD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50539-ACR|
|Mouse CFD ORF mammalian expression plasmid, C-Flag tag||MG50539-CF|
|Mouse CFD ORF mammalian expression plasmid, C-His tag||MG50539-CH|
|Mouse CFD ORF mammalian expression plasmid, C-Myc tag||MG50539-CM|
|Mouse CFD ORF mammalian expression plasmid, C-HA tag||MG50539-CY|
|Mouse CFD Gene cDNA clone plasmid||MG50539-M|
|Mouse CFD ORF mammalian expression plasmid, N-Flag tag||MG50539-NF|
|Mouse CFD ORF mammalian expression plasmid, N-His tag||MG50539-NH|
|Mouse CFD ORF mammalian expression plasmid, N-Myc tag||MG50539-NM|
|Mouse CFD ORF mammalian expression plasmid, N-HA tag||MG50539-NY|
|Mouse CFD natural ORF mammalian expression plasmid||MG50539-UT|
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Mouse complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to the peptidase S1 family. CFD / Adipsin contains one peptidase S1 domain. Complement factor D ( CFD / Adipsin ) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D ( CFD / Adipsin ) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D ( CFD / Adipsin ) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D ( CFD / Adipsin ) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.