|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CADM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50538-ACG|
|Mouse CADM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50538-ACR|
|Mouse CADM1 ORF mammalian expression plasmid, C-Flag tag||MG50538-CF|
|Mouse CADM1 ORF mammalian expression plasmid, C-His tag||MG50538-CH|
|Mouse CADM1 ORF mammalian expression plasmid, C-Myc tag||MG50538-CM|
|Mouse CADM1 ORF mammalian expression plasmid, C-HA tag||MG50538-CY|
|Mouse CADM1 Gene cDNA clone plasmid||MG50538-G|
|Mouse CADM1 ORF mammalian expression plasmid, N-Flag tag||MG50538-NF|
|Mouse CADM1 ORF mammalian expression plasmid, N-His tag||MG50538-NH|
|Mouse CADM1 ORF mammalian expression plasmid, N-Myc tag||MG50538-NM|
|Mouse CADM1 ORF mammalian expression plasmid, N-HA tag||MG50538-NY|
|Mouse CADM1 natural ORF mammalian expression plasmid||MG50538-UT|
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Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression.