|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat EFNB2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80107-ACG|
|Rat EFNB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80107-ACR|
|Rat EFNB2 ORF mammalian expression plasmid, C-Flag tag||RG80107-CF|
|Rat EFNB2 ORF mammalian expression plasmid, C-His tag||RG80107-CH|
|Rat EFNB2 ORF mammalian expression plasmid, C-Myc tag||RG80107-CM|
|Rat EFNB2 ORF mammalian expression plasmid, C-HA tag||RG80107-CY|
|Rat EFNB2 Gene cDNA clone plasmid||RG80107-G|
|Rat EFNB2 ORF mammalian expression plasmid, N-Flag tag||RG80107-NF|
|Rat EFNB2 ORF mammalian expression plasmid, N-His tag||RG80107-NH|
|Rat EFNB2 ORF mammalian expression plasmid, N-Myc tag||RG80107-NM|
|Rat EFNB2 ORF mammalian expression plasmid, N-HA tag||RG80107-NY|
|Rat EFNB2 natural ORF mammalian expression plasmid||RG80107-UT|
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EphrinB2 also known as EFNB2 is a member of the ephrin family. EphrinB2 is involved in establishing arterial versus venous identity and perhaps in anastamosing arterial and venous vessels at their junctions. The transmembrane-associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB2 expression progressively extends from the arterial endothelium to surrounding smooth muscle cells and to pericytes, suggesting that ephrin-B2 may play an important role during formation of the arterial muscle wall.