|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CRABP2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50535-ACG|
|Mouse CRABP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50535-ACR|
|Mouse CRABP2 ORF mammalian expression plasmid, N-GFPSpark tag||MG50535-ANG|
|Mouse CRABP2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50535-ANR|
|Mouse CRABP2 ORF mammalian expression plasmid, C-Flag tag||MG50535-CF|
|Mouse CRABP2 ORF mammalian expression plasmid, C-His tag||MG50535-CH|
|Mouse CRABP2 ORF mammalian expression plasmid, C-Myc tag||MG50535-CM|
|Mouse CRABP2 ORF mammalian expression plasmid, C-HA tag||MG50535-CY|
|Mouse CRABP2 Gene cDNA clone plasmid||MG50535-M|
|Mouse CRABP2 ORF mammalian expression plasmid, N-Flag tag||MG50535-NF|
|Mouse CRABP2 ORF mammalian expression plasmid, N-His tag||MG50535-NH|
|Mouse CRABP2 ORF mammalian expression plasmid, N-Myc tag||MG50535-NM|
|Mouse CRABP2 ORF mammalian expression plasmid, N-HA tag||MG50535-NY|
|Mouse CRABP2 natural ORF mammalian expression plasmid||MG50535-UT|
|Learn more about expression Vectors|
Mouse cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.