|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat VEGFC ORF mammalian expression plasmid, C-GFPSpark tag||RG80103-ACG|
|Rat VEGFC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80103-ACR|
|Rat VEGFC ORF mammalian expression plasmid, C-Flag tag||RG80103-CF|
|Rat VEGFC ORF mammalian expression plasmid, C-His tag||RG80103-CH|
|Rat VEGFC ORF mammalian expression plasmid, C-Myc tag||RG80103-CM|
|Rat VEGFC ORF mammalian expression plasmid, C-HA tag||RG80103-CY|
|Rat VEGFC Gene cDNA clone plasmid||RG80103-G|
|Rat VEGFC ORF mammalian expression plasmid, N-Flag tag||RG80103-NF|
|Rat VEGFC ORF mammalian expression plasmid, N-His tag||RG80103-NH|
|Rat VEGFC ORF mammalian expression plasmid, N-Myc tag||RG80103-NM|
|Rat VEGFC ORF mammalian expression plasmid, N-HA tag||RG80103-NY|
|Rat VEGFC natural ORF mammalian expression plasmid||RG80103-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.