|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat EGFR ORF mammalian expression plasmid, C-GFPSpark tag||RG80100-ACG|
|Rat EGFR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80100-ACR|
|Rat EGFR ORF mammalian expression plasmid, N-GFPSpark tag||RG80100-ANG|
|Rat EGFR ORF mammalian expression plasmid, C-Flag tag||RG80100-CF|
|Rat EGFR ORF mammalian expression plasmid, C-His tag||RG80100-CH|
|Rat EGFR ORF mammalian expression plasmid, C-Myc tag||RG80100-CM|
|Rat EGFR ORF mammalian expression plasmid, C-HA tag||RG80100-CY|
|Rat EGFR Gene cDNA clone plasmid||RG80100-G|
|Rat EGFR ORF mammalian expression plasmid, N-Flag tag||RG80100-NF|
|Rat EGFR ORF mammalian expression plasmid, N-His tag||RG80100-NH|
|Rat EGFR ORF mammalian expression plasmid, N-Myc tag||RG80100-NM|
|Rat EGFR ORF mammalian expression plasmid, N-HA tag||RG80100-NY|
|Rat EGFR natural ORF mammalian expression plasmid||RG80100-UT|
|Learn more about expression Vectors|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.